transwell® permeable supports  (Corning Life Sciences)

Journal: Materials Today Bio

Article Title: Carminic acid/ferric ion self assembled multienzyme mimetic nanodrug with concurrent photothermal/anti-inflammatory activity to prevent breast cancer metastasis

doi: 10.1016/j.mtbio.2025.102028

Figure Lengend Snippet: (A) Representative photos of transwell assay under different conditions. Scale bar: 200 μm. (B) Fluorescence images of H 2 O 2 stimulated 4T1 cells stained with DCF probe after pre-treatment with/without FCP NPs. Scale bar: 20 μm. (C) The level of IL-6 and TNF-α in cell culture supernatant incubated with/without LPS and pre-treated with/without FCP NPs. Data were presented as means ± standard deviations. ∗indicates P < 0.05, ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs LPS group. (D) Study the expression of MMP-9 in 4T1 cells under different concentrations (unit: μg/mL) of FCP NPs by western blotting and (E) the corresponding quantitative analysis. Data were presented as means ± standard deviations. ∗indicates P < 0.05, ∗∗indicates P < 0.01 and ∗∗∗indicates P < 0.001 vs control group.

Article Snippet: Transwell assay: The migration assay were conducted by using 24-well Transwell® permeable supports (Corning, USA) with 10.13039/100022376 FCP NPs (0–400 μg/mL) according to the previous method [ ], and the cells were also photographed with an inverted microscope.

Techniques: Transwell Assay, Fluorescence, Staining, Cell Culture, Incubation, Expressing, Western Blot, Control

Journal: Biomedical Reports

Article Title: Effect of Gracilaria fisheri sulfated galactan with increased sulfation on cell migration and expression of cell adhesion molecules in sodium oxalate-induced HK-2 cell injury

doi: 10.3892/br.2025.2001

Figure Lengend Snippet: Photomicrographs show the effect of SGS on the Transwell migration of HK-2 cells, stained with toluidine blue O at 24 h, with accompanying graphs quantifying the number of migrated cells. Data are presented as mean ± SEM (n=3) from three independent experiments. * P<0.05 vs. normal control; # P<0.05 vs. NaOX control. Scale bar, 500 µm. SGS, sulfated galactan with increased sulfation; NaOX, sodium oxalate; Control, no treatment; NaOX control, treated with 1.25 mmol/l of NaOX; 100-SGS + NaOX, treated with 100 µg/ml of SGS combined with 1.25 mmol/l of NaOX; 1000-SGS + NaOX, treated with 1,000 µg/ml of SGS combined with 1.25 mmol/l of NaOX; Cystone + NaOX, treated with 100 µg/ml of Cystone combined with 1.25 mmol/l of NaOX.

Article Snippet: Transwell permeable supports (6.5 mm inserts, 24-well plates) were purchased from Corning Life Sciences.

Techniques: Migration, Staining, Control

Journal: bioRxiv

Article Title: Canonical microRNA loss drives tumor development implicating therapeutic efficacy of enoxacin in angiosarcoma

doi: 10.1101/2025.07.15.664801

Figure Lengend Snippet: (A) Relative cell viability via Cell Titer Glo in HTHM, ADC106, and SVR cells treated with indicated dose of enoxacin (ENX) for 72 hours. (B) Relative Caspase 3/7 activity in ADC106, SVR, and HTHM cells treated with DMSO or 100 µM ENX for 48 hours. (C) Population doubling assays in ADC106 and SVR cells treated with DMSO or 100µm ENX. (D) Representative images and (E) quantification of clonogenic colony formation in cells treated with DMSO or 100 µM ENX. (F) Representative image and (G) quantification of migrated cells in transwell migration assays in cells treated with DMSO or 100 µM ENX, data presented as the mean of four biological replicate experiments with standard error of mean (SEM), *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Article Snippet: Transwell assays were performed with transwell permeable supports (3422, Corning, Glendale, AZ, USA).

Techniques: Activity Assay, Migration